Distillation of Ethanol

High School Undergraduate
Bioenergy Lab Series
The video above provides a full tutorial of the Distillation of Ethanol lab.

In this lab investigation, students will become familiar with the distillation process using a simple distillation column under the context of ethanol purification.

A fermentation broth will be created or sampled from a previous lab. The broth will then undergo distillation in a simple distillation column. Students will then analyze the purity of the product in terms of ethanol concentration.

Learning Objectives

Upon completion, students will be able to:

  • Understand the relations of volatility and boiling points of substances
  • Purify a compound by separating its components based on their volatility
  • Apply the practice of distillation in the context of ethanol production
  • Identify and discuss the costs and benefits of ethanol distillation as a method of biofuel production
Get the Guide

Fermentation, Distillation, Ethanol Production, Phase Changes, Boiling Points, Vaporization

Background provided

90 minutes

Fermentation broth, yeast, glucose, ethanol, beaker, weigh boats, pipet, graduated cylinder, Brix RI meter, ethanol probe, distillation apparatus, round bottom flasks, 3-way adapter, water jacketed condenser, receiving adapter, Keck clips, stand and clamps